ABSTRACT Two experiments were carried with broilers from 29 to 42 days of age for the nutritional evaluation of dietary chia. Thus, the nitrogen-corrected apparent metabolizable energy (AMEn) and the apparent metabolizability coefficients of gross energy and ether extract of chia seeds and oil, toasted soybean grain (TSG), and soybean oil were determined in the experiment I, by total excreta collection method, using 120 broilers. Each experimental diet (reference diet [RD] and four diets with the tested feedstuffs) was evaluated in eight replicates of three broilers. The TSG and chia seeds replaced the RD in 250 g/kg, whereas soybean and chia oils replaced the RD in 100 g/kg. Simultaneously, a second experiment was carried subdivided into two trials. In the performance trial, we evaluated the dietary feedstuffs effects on performance, carcass and cut yields, blood parameters, and activity of lipogenic enzymes. The nutrient metabolizability coefficients and AMEn were evaluated in the metabolism trial. The AMEn values of 37.49, 37.35, 15.85, and 8.43 MJ/kg of dry matter were determined for chia oil, soybean oil, TSG, and chia seeds, respectively (experiment I). In the second experiment, the best feed conversion was observed in broilers fed diets containing chia oil and TSG. However, the diet formulated with chia seeds worsened broiler feed conversion, exhibited the smaller energy value and apparent metabolizability coefficient of the ether extract, and increased the activity of the malic enzyme and serum total cholesterol level. There was no difference for glucose-6-phosphate dehydrogenase activity and high-density lipoprotein cholesterol level. In general, chia oil showed to be efficient in replacing soybean oil in broiler diets.

Resumo: O objetivo deste trabalho foi comparar o modelo comitê de redes neurais e o modelo de regressão linear múltipla ponderada (RLMP), para estimar a energia metabolizável aparente corrigida por nitrogênio (EMAn) de alimentos para aves. A equação de predição foi ajustada por RLMP e pelo princípio da meta-análise. Os modelos foram comparados tendo-se considerando as percentagens de acerto de predição, com base em intervalos de predição clássicos e intervalos de credibilidade da máxima densidade de probabilidade, e utilizado um teste para comparação de proporções. A acurácia dos modelos foi avaliada com base nos valores de erro médio quadrático, coeficiente de determinação, desvio médio absoluto, erro percentual absoluto médio e viés. Dados provenientes de ensaios metabólicos foram utilizados na comparação dos modelos selecionados. O comitê de redes neurais é o modelo que forneceu predições mais acuradas, sendo recomendado como o de maior acurácia, para prever os valores de EMAn de alimentos concentrados utilizados na indústria alimentícia para aves.

Abstract: The objective of this work was to compare the committee neural network (CNN) and weighted multiple linear regression (WMLR) models, in order to estimate the nitrogen-corrected apparent metabolizable energy (AMEn) of poultry feedstuffs. The prediction equation was adjusted by using a WMLR model and the meta-analysis principle. The models were compared by considering the correct prediction percentages, based on the classic prediction intervals and on the highest-probability density intervals, and by using a comparison test for proportions. The accuracy of the models was evaluated based on the values of the mean squared error, coefficient of determination, mean absolute deviation, mean absolute percentage error, and bias. Data from metabolic trials were used to compare the selected models. The committee neural network is the model that showed the highest accuracy of prediction, being recommended as the most accurate model to predict AMEn values for energetic concentrate feedstuffs used by the poultry feed industry.

Resumo: O objetivo deste trabalho foi determinar a digestibilidade ileal aparente do cálcio e do fósforo em frangos de corte alimentados com rações com diferentes fitases e relações cálcio:fósforo disponível (Ca:Pd). Foram realizados dois experimentos: um com frangos de 22 a 33 dias de idade, e outro com frangos de 35 a 42 dias. As relações Ca:Pd usadas foram de 4,5:1,0, 6,0:1,0 e 7,5:1,0, no primeiro período, e de 3,5:1,0, 5,0:1,0 e 6,5:1,0, no segundo. Todas as dietas foram suplementadas com 1.500 unidades de atividade de fitase por quilograma de seis diferentes fontes de fitase microbiana. Ao final de cada experimento, duas aves por repetição foram abatidas para a coleta do conteúdo ileal. Nas amostras de digestas, foram determinados os teores de Ca e P para posterior cálculo do coeficiente de digestibilidade ileal aparente destes minerais. A relação Ca:Pd influenciou a atividade das fitases, e a maior digestibilidade foi observada nas menores relações, em ambos os experimentos. As fitases que proporcionaram os maiores coeficientes de digestibilidade foram A, D e E. Em ambas as idades avaliadas, a inclusão de fitases às dietas aumentou a digestibilidade de Ca e P, a qual também foi afetada pelo teor de cálcio e pela fonte de fitase utilizada.

Abstract: The objective of this work was to determine the apparent ileal digestibility of calcium and phosphorus in broilers fed diets with different phytases and calcium:available phosphorus (Ca:aP) ratios. Two experiments were carried out: one with broilers with 22 to 33 days of age, and the other with broilers with 35 to 42 days. The Ca:aP ratios used were 4.5:1.0, 6.0:1.0, and 7.5:1.0 in the first period, and 3.5:1.0, 5.0:1.0, and 6.5:1.0 in the second. All diets were supplemented with 1,500 units of phytase activity per kilogram of six different sources of microbial phytase. At the end of each experiment, two broilers per replicate were slaughtered to collect the ileal content. In the samples of digested food, the Ca and P contents were determined for later calculation of the apparent ileal digestibility coefficients of these minerals. The Ca:aP ratio influenced the activity of phytases, and the highest digestibility was observed with lower ratios, in both experiments. The phytases that provided greater digestibility coefficients were A, D, and E. In both ages evaluated, the inclusion of phytases in the feed improved the digestibility of Ca and P, which was also affected by the content of calcium and by the phytase source used.

ABSTRACT The objective of the current study was to evaluate the effects of three calcium:available phosphorus (Ca:aP) ratios and different supplementation with phytases from different origins on performance and ash, Ca, and P contents in the tibia of broilers. A total of 900 male broilers (22 to 35 days old) were fed ten dietary treatments in a completely randomized design. A 3 × 3 + 1 factorial scheme was used, corresponding to three Ca:aP ratios (4.5:1.0, 6.0:1.0, and 7.5:1.0) and three different supplementations with phytases from different origins (isolated or combined supplementation with bacterial and fungal phytase), plus a control diet. Regardless of the dietary Ca:aP ratio, the isolated use of bacterial phytase provided better feed conversion than the fungal phytase but did not differ from combined supplementation with bacterial and fungal phytase. However, regardless of the supplemented phytase, the 7.5:1.0 Ca:aP ratio decreased the feed conversion. Best results for bone P deposition were observed using diets containing the 4.5:1.0 Ca:aP and fungal phytase or the 4.5:1 and 6.0:1 Ca:aP ratios using the bacterial phytase. In general, when the parameters of feed conversion, bone ash, and P content in tibia are considered together, diets containing a 4.5:1.0 or 6.0:1.0 Ca:aP ratio and 1,500 FTU kg−1 bacterial phytase, or a 4.5:1.0 Ca:aP ratio using fungal phytase and only 1.0 g kg−1 available phosphorus provide better results.

Objetivou-se avaliar o desempenho, glicerol sérico e umidade da cama de pintos de corte recebendo rações com diferentes níveis e fontes de glicerina. Foram utilizados 1300 pintos de corte machos Cobb 500, num arranjo fatorial 4x3+1, com delineamento inteiramente casualizados, sendo quatro níveis de inclusão (17,5; 35,0; 52,5 e 70,0g/kg) de três glicerinas (bruta de soja (GS), bruta mista (GM) e semipurificada (GPUR) nas dietas isonutritivas e um tratamento sem glicerina, sendo que para as fontes e níveis de glicerina foram teste de Student-Newman-Keuls e análise de regressão, respectivamente; e para o tratamento, teste de Dunnett. Utilizou-se quatro repetições e 25 aves por parcela. O consumo de ração foi influenciado (P<0,05) pelas fontes de glicerina, sendo a GM a fonte que promoveu menor consumo nas aves, porém, não houve diferença dos tratamentos em relação ao controle. Não houve diferença significativa para o ganho de peso. Houve interação dos níveis e fontes de glicerina para a conversão alimentar. Para a GS, a melhor conversão alimentar com a inclusão de 50,35g; para a GM, houve uma piora linear com o aumento da inclusão de glicerina. Houve interação para a concentração de glicerol sérico nas aves, obtendo aumento linear nas aves que ingeriram a GS e GPUR, e redução linear para a GM. O ganho de umidade da cama foi influenciado pelas fontes de glicerina, sendo que a mista apresentou menor umidade. As glicerinas estudadas podem ser incluídas nas rações para pintos de corte até 70,0g/kg, sem prejudicar o desempenho dos animais.

This work aimed to evaluate the performance, serum glycerol and litter moisture of 1300 male Cobb 500 chicks fed diets containing different levels and sources of glycerin. A 4x3+1 factorial arrangement of treatments in a completely randomized design was used, with four inclusion levels of three glycerine (crude soybean (GS), gross mixed (GM) and semipurified (GPUR)) on isonutrient diets and one control treatment without glycerin. The methods of Student-Newman-Keuls test and regression analysis were applied for sources and levels of glycerin, respectively; and Dunnett test for treatment. It was used four replicates and 25 chickens per pen. Feed intake was affected (P <0.05) by the sources of glycerin, and GM was the source that promoted lower consumption in birds, however, there was no difference in the treatments when compared to control. Also there was no significant difference in weight gain. An interaction of levels and sources of glycerine to the feed conversion was seen.GS had the best feed conversion with the inclusion of 50,35g; GM had a linear worsening with increasing addition of glycerin. There was an interaction for the concentration of serum glycerol in birds, obtaining linear increase in birds consuming the GS and GPUR, and linear decrease for GM. The gain of litter moisture was influenced by the sources of glycerin, with a lower humidity for GM. The glycerine studied may be included in diets for broiler chicks until 70,0g/kg, without harming animal performance.

A utilização de antimicrobianos melhoradores de desempenho na criação de frangos de corte é uma prática comum na indústria avícola e, no Brasil, seu uso deve ser feito de acordo com os limites determinados pelo Ministério da Agricultura Pecuária e Abastecimento (MAPA).Objetivou-se com o presente trabalho determinar o efeito da utilização da bacitracina de zinco, dentro dos níveis recomendados pelo Ministério da Agricultura Pecuária e Abastecimento (MAPA), em dietas para frangos de corte sobre o desempenho de aves e criadas de 1 a 42 dias de idade. Utilizou-se o delineamento inteiramente casualizado, com 5 tratamentos, distribuídos em 10 repetições de 25 pintos machos de corte da linhagem Cobb 500®, totalizando 1250 aves. Os tratamentos experimentais foram constituídos de cinco níveis de inclusão de bacitracina de zinco (0, 10, 25, 40 e 55ppm). As variáveis analisadas foram consumo de ração, ganho de peso, conversão alimentar e avaliação econômica das dietas. Para o período total de criação, avaliou-se também a viabilidade dos frangos de corte. A bacitracina de zinco foi eficiente como beneficiador do desempenho de frangos de corte criados no período de 1 a 21, 1 a 35 e 1 a 42 dias. Para o período de 1 a 21 dias, o uso de 55 e 29,3ppm de bacitracina de zinco resultou em menor consumo de ração e maior ganho de peso, respectivamente. No geral, a melhor conversão alimentar foi obtida quando se adicionou 55ppm de bacitracina de zinco na ração de frangos de corte.

The use of growth promoters in the broilers production is a common practice in the poultry industry and its use must be made accordance with limits in Brazil set by the Ministry of Agriculture, Livestock and Supply (MAPA).The main objective of this study was to verify the effect of the use of zinc bacitracin, within the levels recommended by the Brazilian Ministry of Agriculture, Livestock and Food Supply (MAPA), in broiler diets for the performance of birds from 1 to 42 days old. We used a randomized design with five treatments in 10 replications of 25 male chicks from Cobb cut 500®, totaling 1250 birds. The treatments consisted of five inclusion levels of zinc bacitracin (0, 10, 25, 40 and 55ppm). The variables studied were feed intake, weight gain, feed conversion and economic evaluation of feed. For the breeding period as a whole, this study also evaluated the viability of broilers. Zinc bacitracin was effective as growth promoter of broilers reared during 1 to 21, 1 to 35 and 1 to 42 days. For the period 1-21 days, using 55 and 29.3ppm of zinc bacitracin resulted in a lower feed intake and increased weight gain, respectively. In general, the best feed conversion was obtained when 55ppm of zinc bacitracin was added to the feed of broilers.

O objetivo deste trabalho foi avaliar o uso de valores de equivalência da fitase como base para redução de fósforo disponível (P-disp) em dietas para frangos. Foram avaliados dez planos nutricionais com seis repetições de 15 aves cada uma. Avaliaram-se: desempenho, teor de cinzas na tíbia e custo da ração. De 1 a 7 e de 36 a 42 dias de idade, as aves receberam dieta basal; de 8 a 21 dias, dieta sem fitase contendo 3,9 g kg-1 de P-disp e quatro dietas com fitase contendo de 1,1 a 2,5 g kg-1 de P-disp; e de 22 a 35 dias, dieta sem fitase contendo 3,4 g kg-1 de P-disp mais dieta com fitase contendo 1,6 g kg-1 de P-disp. O melhor plano nutricional foi composto pelas dietas: 4,7 g kg-1 de P-disp sem fitase (1 a 7 dias); 2,0 g kg-1 de P-disp suplementada com fitase (8 a 21 dias); 3,4 g kg-1 de P-disp em ração sem fitase (22 a 35 dias); e 3,0 g kg-1 de P-disp em ração sem fitase (36 a 42 dias). A redução do P-disp ocorre no período de 8 a 21 dias de idade e corresponde a 2,0 g kg-1 de ração suplementada com fitase.

The objective of this work was to evaluate the use of equivalency values of phytase as a basis for reduction of available phosphorus (aP) in broiler diets. Ten nutritional programs were evaluated with six replicates of 15 birds each. The following were evaluated: performance, tibia ash content, and feed costs. From 1 to 7 and 36 to 42 days of age, the birds received basal diet; from 8 to 21 days, diet without phytase containing 3.9 g kg-1 aP and four diets with phytase containing from 1.1 to 2.5 g kg-1 aP; and from 22 to 35 days, diet without phytase containing 3.4 g kg-1 aP plus diet with phytase containing 1.6 g kg-1 aP. The best nutritional plan was formed by the diets: 4.7 g kg-1 aP without phytase (1 to 7 days); 2.0 g kg-1 aP supplemented with phytase (8 to 21 days); 3.4 g kg-1 aP in feed without phytase (22 to 35 days); and 3.0 g kg-1 aP in feed without phytase (36 to 42 days). The reduction of aP occurs in the period from 8 to 21 days of age and corresponds to 2.0 g kg-1 of feed supplemented with phytase.

A redução do teor de fósforo disponível (Pdisp) na dieta de frangos deve ser acompanhada de adequada suplementação da fitase. Um experimento foi conduzido com frangos de corte, no período de 15 a 28 dias de idade, para determinar o nível de fitase necessário quando a ração farelada é formulada com 0,213% de Pdisp para permitir resultados de desempenho, teor de cinzas na tíbia e aproveitamento do cálcio (Ca) semelhantes ao determinado para aves alimentadas com uma dieta formulada para atender suas exigências nutricionais. Desse modo, 120 frangos foram distribuídos em esquema fatorial (4+1) x2, correspondendo a quatro rações deficientes em Pdisp (0,213%) suplementadas com fitase (0; 750; 1.500 ou 2.250FTU kg-1) mais uma ração controle positivo sem fitase (0,426% de Pdisp), fornecidas a frangos machos e fêmeas. Os machos apresentaram melhor desempenho e maior retenção de Ptotal. A redução do teor de Pdisp da dieta sem o uso de fitase piorou o desempenho, o teor de cinzas ósseas e a retenção do Ca, todavia, esses parâmetros foram gradativamente melhorados com o aumento do nível de fitase na ração. Independente do sexo, ao utilizar 2.250FTU kg-1, é possível reduzir o teor de Pdisp para 0,213% sem prejudicar o desempenho, teor de cinzas na tíbia e a retenção do Ca, além de reduzir a excreção do Ptotal em 56,75% e melhorar seu aproveitamento em 38,58%.

The reduction of the available phosphorus (avP) content in the broiler diet must be followed by the supplementation of adequate phytase. One experiment was conducted with broilers from 15 to 28 days of age to determine the level of necessary phytase when the mashed diet is formulated with 0.213% of avP to enable the results of performance, tibia ash content, and calcium (Ca) utilization similar to those determined for birds fed with diet formulated to meet their nutritional requirements. Thus, 120 broilers were distributed in (4+1) x2 factorial arrangement corresponding to four deficient diets in avP (0.213%) supplemented with phytase (0; 750; 1,500 or 2,250FTU kg-1) plus one positive control diet without phytase (0.426% of avP), supplied to male and female broilers. Males showed better performance and higher totalP retention. Decrease in the avP content of the diet without phytase use worsened the performance, tibia ash content, and Ca retention; however, these parameters were improved gradually with the increase of the phytase level in the diet. Regardless of sex, using 2,250FTU kg-1, it is possible to reduce the avP to 0.213% without impairing performance, tibia ash content, and Ca retention; in addition to reducing the totalP excretion in 56.75% and improving its utilization in 38.58%.

Neste estudo, foi proposta a utilização da análise de componentes principais, na formação de grupos homogêneos de artigos científicos, a serem considerados em uma meta-análise. Nessa meta-análise, foram utilizados resultados referentes à composição química e energia metabolizável aparente corrigida pelo balanço de nitrogênio (EMAn) de alimentos para aves, na obtenção de equações de predição da EMAn. Foram considerados 293 experimentos com resultados já publicados. Dados provenientes de ensaios metabólicos foram utilizados na validação das equações de predição obtidas, sendo que estas apresentaram resultados semelhantes às disponíveis na literatura. No procedimento de meta-análise, a formação de grupos homogêneos de resultados experimentais, que é uma das maiores dificuldades, foi facilitada com a utilização de componentes principais, uma vez que não houve a necessidade de determinar variáveis ou fatores a serem considerados nessa classificação. Assim, tem-se uma forma rápida e eficiente de definir tais grupos.

The purpose of this study was to develop a meta-analysis study by using the principal components analysis to obtain homogeneous groups of experimental results. In the process of the meta-analysis, it was considered data from 293 experiments carried out in Brazil. Prediction equations were obtained to estimate the nitrogen-corrected apparent metabolizable energy (AMEn) of poultry feedstuffs. Data from metabolic trials were used to validate the prediction equations obtained, which were similar than other available equations in the literature. One of the problems in meta-analysis is the determination of the homogeneous groups of experiments and, this problem was eliminated by using principal components, since there was no need to establish variables or factors to be considered in this classification.

The objective of this study was to evaluate the effect of reducing the crude protein (CP) with the use of amino acids in diets with the same amount of calories anddifferent levels of soybean oil (SO) for finishing pigs kept in two different ambient temperatures. A total of 120 barrows (initial weight of 68.0±1.9 kg) were utilized for the experiment. The design was of randomized blocks in a 2 × 3 × 2 factorial arrangement (two levels of CP, 16.2 and 13.7%; three levels of SO, 1.5; 3.0 and 4.5%; and two ambient temperatures, 19 and 31 ºC), totaling twelve treatments with five replicates for thirty days. The heat reduced feed intake (FI) of the animals, regardless of the levels of CP and SO used, and reduced weight gain (WG) in diets with reduced CP and SO or higher SO. The increased level of CP and SO improved feed conversion (FC) when the animals were kept in thermoneutral environment and given a diet with higher protein content. The reduction in CP increased FI only in the thermoneutral environment. In the heat, WG increased in diets with higher levels of SO, without influencing FC. The manipulation of diet and temperature did not affect most carcass traits. The SO reduced backfat thickness (BT) only in diets with high CP and animals kept in thermoneutral environment. The reduction of CP increased the BT only in diets with high content of SO, both in hot and thermoneutral environments. Heat reduces FI and the inclusion of SO does not improve performance or carcass characteristics in these conditions. The reduction in CP improves WG only in diets with high oil content; however, it increases BT. In thermoneutral environment, increasing the SO is beneficial for performance and carcass characteristics, except when diets with reduced CP are used. The reduction in CP improves performance only in diets with low oil content.

The experiment was carried out to evaluate the influence of nutritional correction on the partial diet replacement or pure-food methodology to determine the energy values (apparent metabolizable energy - AME and nitrogen-correct apparent metabolizable energy - AMEn) and coefficient of metabolizability of nutrients of maize. The method of total excreta collection was used, with 120 female Cobb 500® broilers weighing 1,339±3 g and at 28 days of age distributed in a completely randomized design with five replicates and four birds per experimental unit. The treatments were pure maize with three levels of nutritional correction (0, 50 and 100%) and a maize-replaced basal diet (400 g/kg) with two levels of correction (0 and 100%). The corrections were made in order to meet the nutritional requirements of calcium, available phosphorus and sodium yonder trace minerals, vitamins, choline, lysine, methionine + cystine, threonine and tryptophan. The AME and AMEn of maize and coefficient of metabolizability of dry matter were higher when correction at 100% was made, regardless of the methodology used (pure feed or replaced basal diet). The correction at 50% of nutrients increased the coefficient of metabolizability of crude protein in pure maize when compared with 0 or 100% of correction. The values determined with pure maize were lower than those determined with the method of partial replacement. The absence of nutritional correction underestimates the energy values and metabolizability of nutrients from maize.

A necessidade de dietas adequadas para frangos, considerando o conceito de proteína ideal associada à fitase, é importante para melhorar a produtividade das aves. Objetivou-se avaliar a necessidade de correção dos níveis de aminoácidos em rações para frangos de corte, formuladas em diferentes combinações de proteína bruta (PB) e suplementadas com fitase. Para o desempenho, foram utilizados 875 pintos de corte Cobb, com oito dias de idade e peso inicial de 155±1,4g. As aves foram distribuídas em delineamento inteiramente casualizado, com sete tratamentos (planos nutricionais) e cinco repetições de 25 aves cada. Para o ensaio de metabolismo, foram selecionadas 300 aves que foram também distribuídas no mesmo delineamento, porém com cinco, quatro e três aves por parcela experimental nas fases de 8 a 21, 22 a 35 e 36 a 42 dias. Os planos nutricionais consistiram em diferentes combinações de PB em rações com cálcio e fósforo reduzidos, suplementadas com fitase e aminoácidos. A redução de aminoácidos em dietas com teores reduzidos de PB aumentou o consumo, a conversão alimentar e a gordura abdominal e diminuiu o rendimento de coxa + sobrecoxa. A inclusão de fitase reduziu a excreção de cálcio e de fósforo, porém aumentou a de nitrogênio quando a PB não foi reduzida. Conclui-se que a redução de aminoácidos em 3,5% em rações para frangos de corte suplementadas com fitase e elaboradas segundo um plano nutricional não melhora o desempenho e as características de carcaça das aves, embora possa reduzir a emissão de elementos poluentes pelas excretas.

The need for suitable diets in chickens, considering the ideal protein concept associated with phytase, is important in order to improve the productivity of birds. The aim of this study was to assess the need for the correction of amino acid levels in the diets of broilers, by using different combinations of crude protein (CP) supplemented with phytase. For performance, eight days old Cobb 875 broiler chicks, with initial body weights of 155 ± 1.4g were used. The birds were distributed in a completely randomised design with seven treatments (nutritional plans) and five replicates of 25 birds. For the metabolism trial, 300 birds were selected which were also distributed using the same design, but with five, four and three birds per experimental stage: 8-21 days, 22-35 days and 36- 42 days. The nutritional plans consisted of different combinations of CP in diets with reduced calcium and phosphorus, supplemented with phytase and amino acids. The reduction of amino acids in diets with reduced levels of CP resulted in an increased intake, feed conversion and abdominal fat level, and decreased the yield of thigh + drumstick. The inclusion of phytase reduced the excretion of calcium and phosphorus, but increased nitrogen excretion when the CP was not reduced. As a result, this study concluded that the reduction of amino acids to 3.5%, supplemented with phytase and prepared according to a nutritional plan does not improve the performance and carcass characteristics of broilers, although it may reduce the emission of polluting elements by excreta.

Um experimento de desempenho e outro de metabolismo foram conduzidos simultaneamente com frangos de corte de 22 a 35 dias de idade, avaliando-se cinco dietas em seis repetições. A dieta controle, sem fitase, continha 19% de proteína bruta (PB); 0,412% de fósforo disponível (Pdisp) e 0,824% de cálcio (Ca). As demais dietas foram suplementadas com fitase e formuladas com 0,262% de Pdisp e 0,524% de Ca, tendo o teor de PB variado em 19 até 16%. No ensaio de desempenho, 750 aves foram utilizadas, avaliando-se o desempenho, o teor de cinzas na tíbia e rendimentos de carcaça, cortes e gordura abdominal, aos 35 dias de idade. O ensaio de metabolismo foi realizado com 90 aves, determinando-se a energia metabolizável aparente, corrigida pelo balanço de nitrogênio e o coeficiente de metabolizabilidade da matéria seca (CMMS) das rações, e também o balanço e retenção de nitrogênio (N) e fósforo (P). As dietas não influenciaram o desempenho, teor de cinzas na tíbia e os rendimentos de carcaça, peito e coxa + sobrecoxa. Entretanto, as aves alimentadas com a ração contendo 16% de PB apresentaram maior teor de gordura abdominal. As rações com 19% de PB apresentaram menor valor energético e CMMS. A retenção de N e P foi maior nas aves que receberam as rações com reduzidos teores de PB e fitase. Portanto, quando a ração de frangos de corte é suplementada com fitase e aminoácidos é possível reduzir a PB, Pdip e Ca em até 16; 0,262 e 0,524%, respectivamente.

One performance experiment and another metabolism experiment were conducted simultaneously with broilers from 22 to 35 days of age, evaluating five diets in six replicates. The control diet, without phytase, contained 19% of crude protein (CP); 0.412% of available phosphorus (aP) and 0.824% of calcium (Ca). The other diets were supplemented with phytase and were formulated with 0.262% of aP and 0.524% of Ca, with the CP level ranging from 19 to 16%. In the performance assay, 750 birds were used, in which were evaluated the performance, the tibia ash content and yields of carcass, cuts, and abdominal fat at 35 days of age. The metabolism assay was conducted with 90 birds, evaluating the apparent metabolizable energy corrected by the nitrogen balance; the metabolizability coefficient of dry matter (MCDM) of the diets, and also the balance and retention of nitrogen (N) and phosphorus (P). Diets did not affect performance, tibia ash content and yields of carcass, breast and thigh + drumstick. However, birds fed diet containing 16% of CP showed greater abdominal fat. Diets containing 19% of CP had the smaller energetic value and MCDM. The retention of N and P was higher in birds fed diets formulated with reduced CP content and phytase. Therefore, when the broiler feed is supplemented with phytase and amino acids it is possible to reduce the CP, aP and Ca for up to 16; 0.262 and 0.524%, respectively.

Em experimentos de desempenho e metabolismo, avaliaram-se os efeitos do fornecimento de rações com níveis reduzidos de proteína bruta, cálcio e fósforo disponível sobre o desempenho, as características de carcaça, o conteúdo de cinzas na tíbia, o balanço e a retenção de nitrogênio e fósforo de frangos de corte nos períodos de 8 a 21 e 8 a 35 dias de idade. Em cada uma destas fases no ensaio de desempenho, foram utilizadas 30 e 25 aves por unidade experimental e, no ensaio de metabolismo, cinco e três aves, respectivamente, em delineamento inteiramente casualizado, com seis repetições. Os tratamentos constituíram-se de rações com 21% proteína bruta sem fitase (controle), formuladas com níveis nutricionais recomendados nas tabelas brasileiras, e rações com 21, 20, 19 e 18% de proteína bruta com fitase (8 a 21 dias) e, no período de 8 a 35 dias, ração com 20% proteína bruta sem fitase (controle), e rações com 20, 19, 18 e 17% de proteína bruta, com fitase. Nas rações com fitase (80 g de fitase - Ronozyme NP(M) por tonelada de ração), o nível de fósforo disponível reduziu em 0,15 e o de cálcio em 0,30 ponto percentual em relação ao da ração controle. Não houve efeito da redução da composição em nutrientes das rações sobre o consumo, o ganho de peso e a conversão alimentar em ambas as fases nem diferença nas características de carcaça aos 35 dias. Todavia, verificou-se maior teor de gordura abdominal quando as aves consumiram ração com o menor nível proteico. A excreção de nitrogênio e fósforo foi menor quando as aves receberam ração com níveis reduzidos desses nutrientes. A porcentagem de cinzas na tíbia, nas fases avaliadas, não diferiu entre os tratamentos. É possível reduzir em até três pontos percentuais o nível proteico das rações, em 0,15 o de fósforo disponível e em 0,30 ponto percentual o de cálcio quando as rações são suplementadas com fitase e aminoácidos.

In performance and metabolism trials, diets with reduced levels of crude protein, calcium and available phosphorus on the performance, carcass characteristics, tibia ashes, balance and retention of nitrogen and phosphorus of broiler chickens in phase from 8 to 21 and 8 to 35 days were evaluated. In each phase, 30 and 25 birds per experimental unit were used in the performance assay and five and three in the metabolism assay, respectively, in a completely randomized experimental design, with six replications. Treatments consisted of diet with 21% crude protein without phytase (control), formulated with nutritional levels recommended on the Brazilian tables, and diets with 21, 20, 19 and 18% crude protein with phytase (8 to 21 days) and from 8 to 35 days, the control diet had 20% crude protein without phytase (control), and others diets with 20, 19, 18, 17% crude protein, with phytase. In the diets with phytase (80 g of Ronozyme NP(M) phytase per ton of diet), the level of available phosphorus was reduced in 0.15 and the calcium in 0.30 percentage points in relation to the control diet. No effect was observed with the reduction of nutrients on feed intake, weight gain, or feed conversion in either phase or for the study of carcass characteristics to the 35 days. However, higher abdominal fat percentage was observed when the broilers were fed diet with lower protein level. The excretion of nitrogen and phosphorus decreased when birds received diets with reduced levels of these nutrients. Ash percentage in the tibia was not influenced by treatments. It is possible to reduce protein level, available phosphorus, and calcium of the diets in up to 3, 0.15 and 0.30 percentage points when the diets are supplemented with phytase and amino acids, respectively.

Dois experimentos independentes, um na fase pré-inicial (1 a 7 dias) e outro na fase inicial (8 a 21 dias de idade), foram realizados com frangos de corte para avaliar níveis de cálcio e fósforo disponível (Pdisp) em rações suplementadas com 500 unidades de atividade de fitase/kg de ração. Cada experimento constou de um ensaio de desempenho e outro de metabolismo e foi conduzido em esquema fatorial (3 × 4) + 1, sendo três níveis de Pdisp (0,42; 0,37; 0,32% na fase pré-inicial e 0,39; 0,34; 0,29% na fase inicial), quatro níveis de cálcio (0,94; 0,84; 0,74; 0,64% na fase pré-inicial e 0,88; 0,78; 0,68; 0,58% na fase inicial) mais uma ração controle. O controle correspondeu à única ração sem fitase e continha 0,47% de Pdisp e 0,94% de cálcio (fase pré-inicial) ou 0,44% de Pdisp e 0,88% de cálcio (fase inicial). Nos ensaios de desempenho, aos 7 e aos 21 dias de idade, foram avaliados o consumo de ração, o ganho de peso, a conversão alimentar e o teor de cinzas na tíbia. Nos ensaios de metabolismo, determinaram-se a energia metabolizável aparente corrigida pelo balanço de nitrogênio (EMAn) das rações e os coeficientes de digestibilidade da matéria seca (CMMS). Na fase pré-inicial, os níveis de cálcio influenciaram o consumo de ração, a EMAn e o CMMS e, na fase inicial, afetaram o conteúdo de cinzas ósseas, a EMAn e o CMMS. Os níveis de Pdisp influenciaram o teor de cinzas na tíbia, a EMAn e o CMMS na fase pré-inicial e as cinzas ósseas e o CMMS na fase inicial. Adicionando 500 unidades de atividade de fitase/kg de ração, é possível reduzir, respectivamente, os níveis de cálcio e Pdisp para 0,64% e 0,37% na fase pré-inicial e 0,58% e 0,29% na fase inicial, pois essa redução não tem efeito negativo sobre o desempenho e a mineralização óssea dos frangos de corte nessas fases.

Two independent experiments, one in pre-starter phase (1 to 7 days) and the other in the starter phase (8 to 21 days of age), were carried out with broilers to evaluate levels of calcium and available phosphorus (aP) in diets supplemented with 500 units of phytase activity/kg of diet. Each experiment consisted of a performance and a metabolic assay, and was conducted in a factorial schedule (3 × 4) + 1, with three aP levels (0.42, 0.37, 0.32% in the pre-starter phase and 0.39, 0.34, 0.29% in the starter phase), four calcium levels (0.94, 0.84, 0.74, 0.64% in the pre-starter phase and 0.88; 0.78, 0.68, 0.58% in the starter phase) plus a control diet. The control corresponded to the only diet without phytase and contained 0.47% of aP and 0.94% of calcium (pre-starter phase) or 0.44% of aP and 0.88% of calcium (starter phase). In the performance assays, at 7 and 21 days of age the feed intake, weight gain, feed conversion and the ash content in the tibia were evaluated. In the metabolic assays the apparent metabolizable energy corrected for nitrogen balance (AMEn) of the feed, and dry matter digestibility coefficients (DMDC) were determined. Calcium levels influenced the feed intake, AMEn and DMDC in the pre-starter phase, and in the starter phase they influenced bone ash, AMEn and DMDC. The aP levels influenced the content of tibia ash, AMEn and DMDC in the pre-starter phase, and bone ash and DMDC in the starter phase. By adding 500 units of phytase activity/kg of diet, it is possible to reduce, respectively, the calcium and aP levels to 0.64% and 0.37% in the pre-starter phase, and to 0.58% and 0.29% in the starter phase, since this reduction does not cause a negative effect on performance or bone mineralization of broilers in the evaluated periods.